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tween 20 pbst  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tween 20 pbst
    Tween 20 Pbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+washes/Tween+20+washing+buffer%2C+1%25+in+PBS/pm42237415-87-11-15
    Average 95 stars, based on 1 article reviews
    tween 20 pbst - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Acute IFN-γ responses drive sensory neuron injury and chronic pain after chikungunya virus infection
    Article Snippet: Samples were then incubated with the primary antibody anti-cleaved caspase-3 (1:1000; Cell signalling Cat: #9661S) for 24 hours at 4 °C. .. After PBS washes, sections were incubated with Alexa FluorTM 488 goat anti-mouse IgG (1:500; Molecular Probes) for 2 h at room temperature, washed, and counterstained with DAPI (Sigma). ..

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
    Article Snippet: Cells were permeabilised with 0.25% (v/v) Triton X-100 (Thermo Fisher Scientific, 85111) in PBS for 10 min at RT and blocked with 2% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3803) for 1 h at RT. .. Cells were incubated with primary antibodies overnight at 4°C , followed by three 15 min PBS washes and incubation with secondary antibodies AlexaFluorTM 488 goat-anti-mouse IgG1 (Invitrogen, A21121) and AlexaFluorTM 568 goat-anti-rabbit (Invitrogen, A11011) at 1:1000 dilution, and DAPI (Sigma-Aldrich, D9542) in a 1:500 dilution, for 1 h at RT. .. The slides were mounted using FluoromountTM (Invitrogen, 00-4958-02).

    Article Title: Loss of ATM causes R-loop–associated transcriptional dysregulation and attenuates the related response to DNA damage
    Article Snippet: Cells were then blocked with 5% normal goat serum (Cole-Parmer, #S-1000) in PBS for 30 min and incubated with primary antibody (1:500 dilution in 5% normal goat serum) overnight at 4 °C. .. After three PBS washes, cells were incubated with fluorescent secondary antibodies (Invitrogen, #A10042, #A11029) for 2 h at room temperature, followed by nuclear staining with 4′,6-diamidino-2-phenylindole (Thermo Fisher, #62248) for 10 min. After three PBS washes, coverslips were mounted onto glass slides using mounting medium (Thermo Fisher, #00-4958-02). .. Images were acquired using an all-in-one Fluorescence Microscope (Keyence and LEICA DMi8), and fluorescence intensity was quantified using ImageJ software (developed by the NIH; https://imagej.net/ij/ ).

    Article Title: Patient-Derived 3D Bioprinted Cardiac Organoid Constructs Reveal Key Pathological Features of Duchenne Muscular Dystrophy.
    Article Snippet: Slides were stained with BODIPY 493/503 4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene (Invitrogen) as previously described (Marini et al., 2022). .. Next, sections were incubated with Hoechst 33 342 diluted 1: 1000 in 1% (w/v) BSA in PBS for 7 min at RT for nuclei staining, which was followed by two PBS washes before mounting the slides with ProLong Gold antifade reagent (Invitrogen). .. Imaging was performed using the Zeiss AxioImager Z1 epifluorescence microscope (Carl Zeiss) with AxioVision SE64 Rel.

    Staining:

    Article Title: BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes.
    Article Snippet: After incubation with the drug for 24 hr, cells were washed with PBS 3 times then fixed with 4% paraformaldehyde (PFA) in 1x PBS (Thermofisher, cat#043368.9M) at room temperature for 15 min in the dark. .. PFA was removed with 3 PBS washes and the fixed cells were staining with Hoechst 33342 (Invitrogen, cat# H3570, final concentration: 5 μg/ml) and HCS CellMask Deep Red Stain (Invitrogen, cat# H32721, final concentration: 1 μg/ml) for 1 hr at 37◦C for 1hr. ..

    Article Title: A missense variant in ASCL5 leads to lobodontia.
    Article Snippet: Following three PBS washes, the cells were incubated for 1 hour with 1:1000 Alexa-488-conjugated secondary antibody (BioLegend, CA, USA) in blocking buffer. .. After another three PBS washes, the cells were stained with 1:1000 rhodamine-phalloidin (Invitrogen, AR TI CL E IN P RE SS MA, USA) for 1 hour, followed by 0.5 μg/ml DAPI staining for 1 minute. .. The cover glasses were then mounted on slides using ProLong Gold Antifade Mountant (Invitrogen, MA, USA).

    Article Title: Identification and structural characterization of anthrax toxin receptor 2 as the Clostridium perfringens NetF receptor.
    Article Snippet: After a PBS wash, cells were permeabilized in 0.2% Triton X-100 in PBS for 10 min, then incubated with 100 nM rhodamine-phalloidin (Cytoskeleton, PHDR1) for 30 min. .. Following three PBS washes, DNA was stained with 0.1 μg/mL DAPI (Invitrogen) for 5 min. ..

    Article Title: Loss of ATM causes R-loop–associated transcriptional dysregulation and attenuates the related response to DNA damage
    Article Snippet: Cells were then blocked with 5% normal goat serum (Cole-Parmer, #S-1000) in PBS for 30 min and incubated with primary antibody (1:500 dilution in 5% normal goat serum) overnight at 4 °C. .. After three PBS washes, cells were incubated with fluorescent secondary antibodies (Invitrogen, #A10042, #A11029) for 2 h at room temperature, followed by nuclear staining with 4′,6-diamidino-2-phenylindole (Thermo Fisher, #62248) for 10 min. After three PBS washes, coverslips were mounted onto glass slides using mounting medium (Thermo Fisher, #00-4958-02). .. Images were acquired using an all-in-one Fluorescence Microscope (Keyence and LEICA DMi8), and fluorescence intensity was quantified using ImageJ software (developed by the NIH; https://imagej.net/ij/ ).

    Article Title: Patient-Derived 3D Bioprinted Cardiac Organoid Constructs Reveal Key Pathological Features of Duchenne Muscular Dystrophy.
    Article Snippet: Slides were stained with BODIPY 493/503 4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene (Invitrogen) as previously described (Marini et al., 2022). .. Next, sections were incubated with Hoechst 33 342 diluted 1: 1000 in 1% (w/v) BSA in PBS for 7 min at RT for nuclei staining, which was followed by two PBS washes before mounting the slides with ProLong Gold antifade reagent (Invitrogen). .. Imaging was performed using the Zeiss AxioImager Z1 epifluorescence microscope (Carl Zeiss) with AxioVision SE64 Rel.

    Concentration Assay:

    Article Title: BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes.
    Article Snippet: After incubation with the drug for 24 hr, cells were washed with PBS 3 times then fixed with 4% paraformaldehyde (PFA) in 1x PBS (Thermofisher, cat#043368.9M) at room temperature for 15 min in the dark. .. PFA was removed with 3 PBS washes and the fixed cells were staining with Hoechst 33342 (Invitrogen, cat# H3570, final concentration: 5 μg/ml) and HCS CellMask Deep Red Stain (Invitrogen, cat# H32721, final concentration: 1 μg/ml) for 1 hr at 37◦C for 1hr. ..



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