tween 20 pbst (Thermo Fisher)

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Thermo Fisher tween 20 pbst
Tween 20 Pbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbs+washes/Tween+20+washing+buffer%2C+1%25+in+PBS/pm42237415-87-11-15
Average 95 stars, based on 1 article reviews

tween 20 pbst - by Bioz Stars, 2026-10

95/100 stars

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Incubation:

Article Title: Acute IFN-γ responses drive sensory neuron injury and chronic pain after chikungunya virus infection
Article Snippet: Samples were then incubated with the primary antibody anti-cleaved caspase-3 (1:1000; Cell signalling Cat: #9661S) for 24 hours at 4 °C. .. After PBS washes, sections were incubated with Alexa FluorTM 488 goat anti-mouse IgG (1:500; Molecular Probes) for 2 h at room temperature, washed, and counterstained with DAPI (Sigma). ..

Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
Article Snippet: Cells were permeabilised with 0.25% (v/v) Triton X-100 (Thermo Fisher Scientific, 85111) in PBS for 10 min at RT and blocked with 2% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3803) for 1 h at RT. .. Cells were incubated with primary antibodies overnight at 4°C , followed by three 15 min PBS washes and incubation with secondary antibodies AlexaFluorTM 488 goat-anti-mouse IgG1 (Invitrogen, A21121) and AlexaFluorTM 568 goat-anti-rabbit (Invitrogen, A11011) at 1:1000 dilution, and DAPI (Sigma-Aldrich, D9542) in a 1:500 dilution, for 1 h at RT. .. The slides were mounted using FluoromountTM (Invitrogen, 00-4958-02).

Article Title: Loss of ATM causes R-loop–associated transcriptional dysregulation and attenuates the related response to DNA damage
Article Snippet: Cells were then blocked with 5% normal goat serum (Cole-Parmer, #S-1000) in PBS for 30 min and incubated with primary antibody (1:500 dilution in 5% normal goat serum) overnight at 4 °C. .. After three PBS washes, cells were incubated with fluorescent secondary antibodies (Invitrogen, #A10042, #A11029) for 2 h at room temperature, followed by nuclear staining with 4′,6-diamidino-2-phenylindole (Thermo Fisher, #62248) for 10 min. After three PBS washes, coverslips were mounted onto glass slides using mounting medium (Thermo Fisher, #00-4958-02). .. Images were acquired using an all-in-one Fluorescence Microscope (Keyence and LEICA DMi8), and fluorescence intensity was quantified using ImageJ software (developed by the NIH; https://imagej.net/ij/ ).

Staining:

Article Title: BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes.
Article Snippet: After incubation with the drug for 24 hr, cells were washed with PBS 3 times then fixed with 4% paraformaldehyde (PFA) in 1x PBS (Thermofisher, cat#043368.9M) at room temperature for 15 min in the dark. .. PFA was removed with 3 PBS washes and the fixed cells were staining with Hoechst 33342 (Invitrogen, cat# H3570, final concentration: 5 μg/ml) and HCS CellMask Deep Red Stain (Invitrogen, cat# H32721, final concentration: 1 μg/ml) for 1 hr at 37◦C for 1hr. ..

Article Title: A missense variant in ASCL5 leads to lobodontia.
Article Snippet: Following three PBS washes, the cells were incubated for 1 hour with 1:1000 Alexa-488-conjugated secondary antibody (BioLegend, CA, USA) in blocking buffer. .. After another three PBS washes, the cells were stained with 1:1000 rhodamine-phalloidin (Invitrogen, AR TI CL E IN P RE SS MA, USA) for 1 hour, followed by 0.5 μg/ml DAPI staining for 1 minute. .. The cover glasses were then mounted on slides using ProLong Gold Antifade Mountant (Invitrogen, MA, USA).

Article Title: Identification and structural characterization of anthrax toxin receptor 2 as the Clostridium perfringens NetF receptor.
Article Snippet: After a PBS wash, cells were permeabilized in 0.2% Triton X-100 in PBS for 10 min, then incubated with 100 nM rhodamine-phalloidin (Cytoskeleton, PHDR1) for 30 min. .. Following three PBS washes, DNA was stained with 0.1 μg/mL DAPI (Invitrogen) for 5 min. ..

Concentration Assay:

other:

Article Title: Patient-Derived 3D Bioprinted Cardiac Organoid Constructs Reveal Key Pathological Features of Duchenne Muscular Dystrophy.
Article Snippet: Slides were stained with BODIPY 493/503 4,4-Difluoro-1,3,5,7,8Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene (Invitrogen) as previously described (Marini et al., 2022).

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